de clean  (GE Healthcare)

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Toona sinensis Leaf Aqueous Extract Improves the Functions of Sperm and Testes via Regulating Testicular Proteins in Rats under Oxidative Stress

doi: 10.1155/2012/681328

Figure Lengend Snippet: 2-DE gel analysis of protein expression in testes of rats treated with H 2 O 2 and H 2 O 2 plus TSL-A. Protein expressions in testes of SD rats IP injected with 1 mmol H 2 O 2 /kg b.w every other day (a) and fed with normal diet, or TSL-A (13 mg/kg b.w/day) (b) for 8 weeks, were separated by 2-DE. Protein spots with numbers represented the seven-protein expression more than 2-fold analyzed by the ImageMaster 2D Platinum Software. Details of the proteins were given in .

Article Snippet: The 2-DE reagent including acrylamide solution (25%) thiourea, immobiline dry strips, immobilized pH gradients (IPG) buffer (pH = 3–10), IPG cover mineral oil, iodoacetamide (IAA), TEMED, trifluoroacetic acid (TFA), 2-DE clean-up kit, 2-DE Quant kit, and silver staining kit were purchased from GE healthcare (Piscataway, NJ, USA).

Techniques: Expressing, Injection, Software

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Toona sinensis Leaf Aqueous Extract Improves the Functions of Sperm and Testes via Regulating Testicular Proteins in Rats under Oxidative Stress

doi: 10.1155/2012/681328

Figure Lengend Snippet: Alterations of 7 identified protein expressions from 2-DE gel. Protein expressions in testes of SD rats IP injected with 1 mmol H 2 O 2 /kg b.w every other day and fed with normal diet and TSL-A (13 mg/kg b.w/day) for 8 weeks were separated by 2-DE. Protein spots with numbers represented the 7-protein expression more than 2-fold analyzed by the ImageMaster 2D Platinum Software. Details of the proteins were given in . The symbols “+” and “−” represent the upregulation and downregulation of the protein expression, respectively. The expression fold was that of the H 2 O 2 group.

Article Snippet: The 2-DE reagent including acrylamide solution (25%) thiourea, immobiline dry strips, immobilized pH gradients (IPG) buffer (pH = 3–10), IPG cover mineral oil, iodoacetamide (IAA), TEMED, trifluoroacetic acid (TFA), 2-DE clean-up kit, 2-DE Quant kit, and silver staining kit were purchased from GE healthcare (Piscataway, NJ, USA).

Techniques: Injection, Expressing, Software

Journal: PLoS ONE

Article Title: Pre-Absorbed Immunoproteomics: A Novel Method for the Detection of Streptococcus suis Surface Proteins

doi: 10.1371/journal.pone.0021234

Figure Lengend Snippet: One sample of the S. suis -derived antiserum was pre-absorbed with whole-cell S. suis to remove the antibodies that recognize outer surface proteins (“pre-absorbed”). Then, untreated and “pre-absorbed” antisera were used to probe 2-DE gels of S. suis proteins. Spots that appear in the blot probed with untreated antiserum, but that were absent from the blot probed with “pre-absorbed” antiserum, were evaluated to identify the surface proteins.

Article Snippet: Prior to rehydration, the precipitated proteins were treated using a 2-DE Clean-up kit (GE Healthcare) to remove contaminants that can interfere with electrophoresis.

Techniques: Derivative Assay

Journal: PLoS ONE

Article Title: Pre-Absorbed Immunoproteomics: A Novel Method for the Detection of Streptococcus suis Surface Proteins

doi: 10.1371/journal.pone.0021234

Figure Lengend Snippet: (A) HA9801 total cell proteins (pH 4–7, 13 cm), stained with colloidal Coomassie brilliant blue G-250. (B) 2-DE blot of S. suis stained with Ponceau S. (C) 2-DE blot of S. suis proteins probed with untreated antiserum. (D) 2-DE blot of S. suis proteins probed with “pre-absorbed” antiserum.

Article Snippet: Prior to rehydration, the precipitated proteins were treated using a 2-DE Clean-up kit (GE Healthcare) to remove contaminants that can interfere with electrophoresis.

Techniques: Staining

Journal: Proteome Science

Article Title: 2-DE analysis indicates that Acinetobacter baumannii displays a robust and versatile metabolism

doi: 10.1186/1477-5956-7-37

Figure Lengend Snippet: 2-DE gels showing A. baumannii proteins . (A) membrane proteins, (B) cytoplasmatic proteins. Numbered spots (in A) indicate membrane proteins identified by MALDI-TOF/TOF. All gels (12% SDS) were silver stained and loaded with 25 μg total protein.

Article Snippet: Finally, as with the soluble fraction, the extract was processed with a 2-DE Cleanup Kit (GE Healthcare, USA).

Techniques: Staining

Journal: Proteome Science

Article Title: 2-DE analysis indicates that Acinetobacter baumannii displays a robust and versatile metabolism

doi: 10.1186/1477-5956-7-37

Figure Lengend Snippet: The cytoplasmatic fraction was analysed with IPG strips of various pH range and silver stained gels with different concentrations of acrylamide . 2-DE (12% SDS) gels containing proteins within a range pH 4-7 (A), 2-DE (15% SDS) gel containing proteins within a range pH 3-10 (B), gels were loaded with 25 μg, total protein. Numbered spots indicate proteins identified by MALDI-TOF/TOF.

Article Snippet: Finally, as with the soluble fraction, the extract was processed with a 2-DE Cleanup Kit (GE Healthcare, USA).

Techniques: Staining, SDS-Gel

Journal: Proteome Science

Article Title: 2-DE analysis indicates that Acinetobacter baumannii displays a robust and versatile metabolism

doi: 10.1186/1477-5956-7-37

Figure Lengend Snippet: 2-DE (12% SDS) gel containing basic proteins within a pH range of 6-11 . Gel was loaded with 40 μg, total protein. Numbered spots indicate proteins identified by MALDI-TOF/TOF.

Article Snippet: Finally, as with the soluble fraction, the extract was processed with a 2-DE Cleanup Kit (GE Healthcare, USA).

Techniques: SDS-Gel